The purpose of this study is to see if the experimental HIV vaccine pGA2/JS2 is safe and is well tolerated at two different doses. Another important purpose of this study is to observe how the immune system responds to the vaccine at different dose levels. Vaccines are given to people to help their bodies fight infection. The vaccine being tested in this study is a DNA vaccine. The pGA2/JS2 plasmid DNA vaccine instructs the body to make some HIV proteins. These HIV proteins may trigger an immune response. Because only a few of the many proteins HIV needs are made through DNA vaccination, there is no risk of getting HIV from the vaccination. This and other similar DNA vaccines have been tested for safety in mice, rabbits, and monkeys. The vaccine has been well tolerated at doses to be used in this study.
Inclusion Criteria Participants may be eligible for this study if they: * Are between the ages of 18 and 40. * Are at low risk of HIV infection. * Have access to a participating study site and are available for follow-up for 12 months. * Complete a questionnaire to evaluate understanding of the study prior to enrollment. * Are willing to receive HIV test results. * Are in good general health. * Do not have hepatitis B. * Are HCV antibody negative or, if HCV antibody positive, are HCV PCR negative. * Have had a negative HIV blood test within 8 weeks prior to enrollment. * Women of childbearing potential must agree to use acceptable methods of contraception. * Note: All inclusion criteria must be assessed within 56 days prior to study entry. Exclusion Criteria Participants may not be eligible for this study if they: * Have been immunized against smallpox. * Have received HIV vaccines or placebo in a previous HIV vaccine trial. * Have used drugs that interfere with the immune system within the past 6 months. * Have received blood products within 120 days before HIV screening. * Have received immunoglobulin within 60 days before HIV screening. * Have received a live vaccine within 30 days prior to initial study vaccine administration. * Have used investigational research agents within 30 days prior to initial study vaccine administration. * Have received a killed vaccine or allergy treatment injections within 14 days of study vaccine administration. * Are currently taking anti-TB therapy. * Have a history of serious harmful reactions to vaccines. * Have a history of immune system disease. * Have a history of unstable asthma. * Have a history of type I or type II diabetes. * Have a history of thyroid disease. * Have a history of tissue swelling with serious episodes. * Have a history of high blood pressure. * Have a history of a bleeding disorder that was diagnosed by a doctor. * Have active syphilis. * Have a history of cancer, unless it has been surgically removed and in the opinion of the investigator is not likely to recur during the study period. * Have a history of a seizure disorder. * Have had their spleen removed. * Have mental illness that would interfere with compliance with the protocol. * Have any other conditions that, in the judgement of the investigator, would interfere with the study. * Are pregnant or breast-feeding. * Note: All exclusion criteria must be assessed within 56 days prior to study entry.